Roles of OsPT6 on the Phosphate Uptake and Utilization in the Rice Seeding of Wuyujing 7

doi:10.3969/j.issn.1001-7216.2014.01.001

Abstract

Abstract: As one of members of the Pht1 transporters family in rice,the phosphate transporter OsPT6 plays a broad role in Pi uptake, translocation and internal transport throughout the plant. With the OsPT6 overexpressed transgenic rice of Wuyujing 7, a highyielding variety, we studied the roles of OsPT6 on the phosphate uptake and utilization in rice. The main results are the following: 1)OsPT6 is abundantly expressed in roots of Wuyujing 7. Furthermore, the expression levels of OsPT6 in shoots and roots are induced by phosphate deficiency. 2)The overexpression of OsPT6 can promote the growth of plants. The dry weight of shoots and roots of the OsPT6 overexpressed transgenic plants was significantly increased, in both sufficient and deficientPi conditions for 35 days. 3)The total P concentration increases in different tissues and organs of OsPT6 overexpressed transgenic plants, especially in vegetable organs, at different Pisupply levels.

Key words: rice, phosphate transporter, overexpression

摘要： 磷转运蛋白OsPT6为水稻Pht1家族成员之一，具有吸收和转运磷酸盐双重功能。以水稻高产品种武育粳7号的OsPT6超表达转基因材料为试材，研究磷转运蛋白OsPT6在武育粳7号磷素吸收利用中的作用。结果表明：1)OsPT6在武育粳7号的地下部表达丰度较高，同时在地下部和地上部该基因均受缺磷诱导表达量显著增加。2)OsPT6超表达后，转基因植株生长良好。正常供磷和低磷处理35 d后,OsPT6超表达植株地上部和地下部干物质量都显著增加。3)OsPT6超表达转基因材料在不同浓度磷素处理后，各组织全磷含量有所增加，其中营养器官尤为显著。

关键词: 水稻, 磷转运蛋白, 超表达

CLC Number:

ZHANG Fang#, AI Hao#, WANG Danfeng, CAO Yue, SHI Shulin, GU Mian, SUN Shubin*, XU Guohua. Roles of OsPT6 on the Phosphate Uptake and Utilization in the Rice Seeding of Wuyujing 7[J]. Chinese Journal of Rice Science, 2014, 28(1): 1-8.

张芳#,艾昊#,王丹凤,曹越,史书林,顾冕,孙淑斌*,徐国华. 磷转运蛋白OsPT6在水稻武育粳7号苗期磷素吸收利用中的作用[J]. 中国水稻科学, 2014, 28(1): 1-8.

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